By Daniel L. Purich
The Advances in Enzymology and comparable parts of Molecular Biology Series is likely one of the so much prestigious within the box, dedicated to the most recent developments in molecular biology and enzymology. each one quantity of the sequence comprises contributions from best gurus within the box. lower than Dr. Purich's editorship, which all started with quantity seventy two, the sequence has multiplied its assurance to incorporate thematic volumes targeting particular study parts, in addition to non-thematic volumes which include chapters with a extra normal allure.
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Attention was focussed on this fixation particularly by the experiments of Evans (79), who found that pyruvate is oxidized by pigeon liver even in the presence of malonate, and is converted to C, dicarboxylic acids, a-ketoglutaric acid and carbon dioxide. No theory was advanced by Evans (79) to explain the mechanism but viewed in the light of the experiments of Krebs and Eggleston (80) on pyruvate oxidation by pigeon breast muscle, it waa evident that pigeon liver very likely possessed a malonate-insensitive mechanism for formation of C4 dicarboxylic acids and dissimilated pyruvate by the Krebs cycle.
Actually the quantitative data of Wood et al. (14), indicate that the succinate formed in the glycerol fermentation contains only one k e d carbon atom. This fact is a strong argument against any scheme that implies a part of the succinate is to contain two fixed carbons. The evidence supporting the contention that there is approximately only one fixed carbon in each succinate molecule has been arrived a t by the following calculation. It has been assumed that the concentration of CL3in the carbon dioxide fixed by the cell is equal to that of the medium at the conclusion of the fermentation.
Under the circumstances it is necessary to study the mechanism of the reaction before use. Much experimental work is needed in this field before the full benefits of isotopes can be realized. With the position of the fixed carbon definitely shown to be in the carboxyl group of the propionate, the problem of the mechanism of fixation can be considered more accurately. (71),and Krebs and Eggleston (44)for formation of propionate containing fixed carbon: 30 CHrOH CHOH WERKMAN AND WOOD - . COOH CO &OH COOH +4H COOH .