Biomembranes Part V: Cellular and Subcellular Transport: by John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca

By John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca Fleischer

The delivery volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of equipment in Enzymology, which lined shipping in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 persevered the subject matter with ATP-Driven Pumps and comparable shipping. mobile and Subcellular delivery: Eukaryotic (Nonepithelial) Cells was once the subject of Volumes 173 and 174. The subject matter of this quantity, in addition to of quantity 192, is mobile and Subcellular shipping: Epithelial Cells

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Additional resources for Biomembranes Part V: Cellular and Subcellular Transport: Epithelial Cells

Example text

A2. 2 for the determination of the shunt conductance under the constraint that I - V relations of all cell membranes are linear and zero-current potentials constant. In this method the resistance of the apical cell membrane R i is varied and the shunt conductance G~-- 1/R,h is derived from a plot of the transepithelial conductance (GT) versus the short circuit current (I,~). In particular we address the following questions. 1. Under what conditions is the relationship between GT and I~ linear? What does this imply for the applicability of the method?

BI. Model of the epi~efiura wi~ two distinct apical permeation pathways. For details see text. Case A corresponds to k = 0 (the ionophore transfers only ion 1, but R~ does not change). Case B corresponds to 0 < k < 1 (the ionophore also has a small selectivity for ion 2; however, Ral and Ra2 change differently). ~ are lowered by the same factor x). We now consider the general case and derive GT and I~ for the equivalent circuit of Fig. B 1. h --- xRal + [1 + k(x - 1)]R~ + G,h (B1) x[1 + k(x - 1)]R,,R,a + Rb~(xR,, + [1 + k(x - 1)]R~2) where Gel is the conductance of the transcellular permeation pathway.

The advantage of the method is that it does not rely on unproved assumptions such as constancy of zero-current potentials, which is a precondition for most of the other methods discussed below. On the other hand, the method has limited power, because it is based on the "lumped" model equivalent circuit. The problem manifests itself in the use of the voltage divider ratio, ct, which is taken to equal RJRb~, while in fact it may be lower ff Rt~ was overestimated due to a significant contribution of the lateral space resistance, Ru~.

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