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8 g for both sexes (see Table I). 6 g Ca/day, suggested by the Academy and Council, during the first 12 months of life, appears to be adequate, but the source of the calcium is an important factor. , Burke et al, 1962) and are certainly adequate. They are, however, far higher than those calculated by Mitchell et al (1945) or Irving (1950b) from known daily calcium increments in the body. D URING P REGN ANCY Since the products of conception at term contain at most only about 25 g of calcium, very little extra calcium need be given to supply this amount.
More recently Haddad and Chyu (1971) reported that 85% of 25-HCC was bound to an inter-ar-globulin, and 10% was associated with albumin. The molecular weight of the binding protein was 40,000-50,000, and cholecalciferol and ergocalciferol were less competitive in protein binding. Mawer and Stanbury (1968) gave labeled cholecalciferol intravenously to humans. They found that the radioactivity of a polar metabolite in the blood rose from zero and reached a maximum within 7-14 days, and then decayed with a long half-life.
Although all the animals grew at the same rate and looked equally healthy, those on the higher calcium intake contained more calcium during the growth period, the difference gradually disappearing as the rats became middle-aged. 35% calcium till 50 days later. Fairbanks and Mitchell (1936) also found that the carcass calcium increased with high calcium contents of the diet. It thus appears that the total calcium content of the body can be increased by extra calcium in the diet during growth even though the growth rate and appearance of the animals are unaffected.