Cell and Tissue Culture Models in Dermatological Research by M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd,

By M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd, Professor Dr. Jürgen Bereiter-Hahn, Priv.-Doz. Dr. Frank Hevert, Professor Dr. H. Holzmann (eds.)

In this quantity the effect of cellphone tradition versions on dermatological learn is mentioned via scientists from medication, body structure, biology, pharmacology,and pharmacy. The publication contains the next subject matters: orthology and pathology of the outside, differentiation of keratinocytes and fibroblasts, difficulties of the cultivation of melanocytes, biochemistry of pigment metabolism, versions for wound therapeutic and tissue renewal, fibroblast functionality and metabolism of collagen, versions for the research of getting older in addition to types for pharmacological and toxicologial assessments. therefore, a large arch is spanned, from easy issues of cultivation and characterization of phone cultures to examples of program. Dermatologists in addition to mobile biologists will make the most of this publication.

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Frenz G, Moller U, Christensen IB (1980) J Invest Dermatol 74:119 3. Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation associated human nuclear antigen defined by monoclonal antibody Ki-67. Immunol133:1710 4. Gormar FE, Bernd E, Bereiter-Hahn J, Holzmann H (1990) A new model of epidermal differention induction by mechanical stimulation. Arch Dermatol Res 282:22 5. Klaus G, Merke J, Eing H, HUgel U, Milde P, Reichel H, Ritz E, Mehls 0 (1991) 1,25(OH) D3 receptor regulation and 1,25 (OH2) D3 effects in primary cultures of growth cartilage of the rat.

4H, griseofulvin treatment induced the cell-surface staining and strongly enhanced the amount of La protein deposited to the extracellular matrix. Similar alterations were also observed for mercury chloride [5], which is known to cause autoantibody production in mice. Such conditions could convert nonpathogenic to pathogenic antibodies. Conclusions Taken together, our cell culture system allows us to test if sera contain pathogenic ANAs and why these ANAs are or become pathogenic. In addition, our cell culture system allows us to test whether a drug induces or protects against autoimmune reactions: Pretreatment of cultured cells with chloroquine - a drug which is used to treat autoimmune patients - followed by a treatment with griseofulvin protected the cells against antibody binding (not shown).

Experiments with buccal Cells Results are shown in Table 2. The yeast strain from the patient with seborrhoic dermatitis (6216 S) adhered in all cases less than the strain (6878 N; a < 1%) from the healthy donators. Results with cells from each single donator varied from day to day significantly. Yeast cells (both strains) adhered to buccal cells from seborrhoic as well as from healthy donators. The number of available donators decreased from 9 to 2 over 4 days. Table 2. Adherence of Candida albicans to freshly exfoliated buccal cells of seborrhoic patients (S l-S 6) and healthy donators (N 1-N 3) means of six countings given as yeast cells/1000 buccal cells Donator Yeast Adherent yeast cells/100 buccal cells day 1 day 2 day 3 day 4 Sl S N S N S N S N S N S N S N S N S N 90 250 41 S2 S3 S4 S5 S6 N1 N2 N3 72 10 26 31 159 54 136 29 229 43 118 27 96 38 84 43 189 10 17 26 164 10 55 61 112 10 48 18 39 19 39 8 53 32 121 39 81 31 148 50 102 35 88 41 73 N, 6878 N intestine isolate; S, 6216 S intestine isolate Discussion Adhesion to Human Intestine Cells Numerous authors have investigated the influence of serum on the adhesion process [17].

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