Cell Culture in the Neurosciences by Jane E. Bottenstein (auth.), Jane E. Bottenstein, Gordon

By Jane E. Bottenstein (auth.), Jane E. Bottenstein, Gordon Sato (eds.)

A basic challenge in neuroscience is the elucidation of the mobile and molecular mechanisms underlying the improvement and serve as of the fearful method. The complexity of association, the heteroge­ neity of phone forms and their interactions, and the trouble of controlling experimental variables in intact organisms make this an impressive job. as a result of skill that it presents to investigate smaller parts of the fearful process (even unmarried cells every now and then) and to raised keep an eye on experimental variables, mobilephone tradition has turn into an more and more invaluable instrument for neuroscientists. Many features of neural improvement, reminiscent of proliferation, differentiation, synaptogenesis, and myelination, take place in tradition with time classes remarkably just like these in vivo. therefore, in vitro tools frequently offer very good version structures for investigating neurobiological questions. Ross Harrison defined the 1st tradition of neural tissue in 1907 and used morphological ways to study the cultures. considering the fact that that point the process has been gradually transformed and used to handle an ever widening diversity of developmental questions. in recent times a con­ vergence of latest or stronger cellphone tradition, biochemical, electrophysiol­ ogical, and immunological tools has happened and been dropped at undergo on neurobiological questions. This quantity is meant to not be accomplished yet really to focus on a number of the newest findings, with a overview of prior very important paintings besides, during which combos of those tools are used.

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1982. Mitogens for glial cells: A comparison of the response of cultured astrocytes, oligodendrocytes and Schwann cells, Develop. Brain Res. 2:19-35. , 1979, Cell-type specific markers for distinguishing and studying neurons and the major classes of glial cells in culture, Brain Res. 174:283-308. , 1983, Localization of insulin-like immunoreactivity in the neurons from primary cultures of rat brain, Exp. Cell Res. 143:351-357. , 1982, Increase in circulating basement membrane antigens in diabetic rats and effects of insulin treatment, Diabetologia 23:266-269.

Future Directions The mechanisms of action of hormones, growth factors, neurotransmitters, and neuromodulators can be more easily investigated using rigorously defined culture conditions. In addition, the potential for identifying new regulatory factors is increased when undefined substances are absent from the culture environment. Further clarification of the role Growth of Neural Cells in Defined Media 35 of endogenous factors and heterotypic cell-cell interactions is possible when isolated populations of particular cell types and their recombinations are used in conjunction with defined culture conditions.

1981). Chapter 9 gives more details about the neuronal characteristics of these differentiated cells. The 1003 cells remain undifferentiated when maintained at subconfluent levels in serum-containing medium (Fig. 13A), but begin to differentiate 1 day after exposure to defined medium (Fig. 13B) and subsequently form interconnected clusters of neurite-bearing neurons (Fig. 13C). Figure 14 shows at higher magnification the extensive radial outgrowth in defined medium of neurites from a cluster of these neurons Figure 13.

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