Culture of Marine Invertebrate Animals: Proceedings — 1st by John M. King (auth.), Walter L. Smith, Matoira H. Chanley

By John M. King (auth.), Walter L. Smith, Matoira H. Chanley (eds.)

This quantity relies on prec'entations on the convention on tradition of Marine Invertebrate Animals which was once held in eco-friendly­ port, ny in October, 1972. The convention used to be subsidized through the center Atlantic normal Sciences Council, Inc., a non­ revenue academic company, including the Marine technological know-how facilities of Adelphi collage, the country collage of latest York at Stony Brook, ny collage, Suffolk County neighborhood university, and the preserve Island Oyster corporation. the aim of the convention used to be to supply a wanted ex­ swap of information between scientists of assorted specialties whose info will be useful to others faced with comparable difficulties, in spite of diversified marine animals. half I considers supportive concepts -- basic isolation and tradition tools, difficulties of illness and feeding. particular options hired within the tradition of quite a lot of invertebrate organisms is roofed partially II. we wish to thank the individuals for his or her cooperation in getting ready the manuscripts in accordance with their convention shows. Walter L. Smith Matoira H. Chanley v Contents half I Recirculating method tradition tools for Marine Organisms .............•.

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S3 PHYTOPLANKTON FOR FEEDING MARINE INVERTEBRATES Vitamin B12 is similarly obtained as crystals and ll% should be allowed for water of crystallization. Weigh (or buy in weighed amounts) and make a primary stock solution having 1 mg/ml. Acidify the solution if it is to be aut 0 claved, and keep sterile and frozen. Primary stocks can be put up in ampoules or small screw-capped test tubes. 1 ml of B-12 primary stock to 100 ml and add 20 mg of thiamine HCl. No primary stock of thiamine is needed. The vitamin stock solution is dispensed in 1, 2, or 5 ml lots in ampoules or in 10 ml lots in screw-capped test tubes, autoclaved, then stored in a refrigerator.

Most of the successful algal species belong to one of six algal classes. (There are some 15 algal classes according to the system of Christensen. ) The six classes referred to, and representative useful species, are: Class Bacillariophyceae, the diatoms: Skeletonema costatum, Tbalassiosira pseudonana (ex Cyclotella nana), Thalassiosira £luviatilis, Phaeodactylum tricornutum, CEaetoceros calcitrans, Chaetoceros simplex, Ditylum brightwellii. Class Haptophyceae, golden brown flagellates (similar to the class Chrysophyceae mentioned next): Isochrysis galbana, Dicrateria inornata, Crisosphaera carterae, Coccolithus huxleyi.

Tubes are available commercially that have ~uite different spectral emissions. Tubes with certain of these spectral characteristics were developed to match the absorption spectrum of green plants, or to match the appearance of daylight to the human eye. However, there is no definite evidence that such tubes are better for growing marine algae, most of which in fact are golden-brown in color and well adapted to absorbing the bluish-green light that has maximum penetration in clear seawater. Manufacturers use different names to describe the spectral properties of their bulbs.

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