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1991). Mab Developmental (raised against hind limb muscle of 7 days old rats (Novocastra Labs. , Newcastle upon Tyne, UK) recognises a Developmental type MHC present during the embryonic and neonatal period in the development of muscle (Butler-Browne et al. 1988). Immunohistochemical staining Transverse serial sections (10 µm) were made with a cryostat at - 20°C. Overnight fixation took place at -20°C in a 35% methanol, 35% acetone and 5% acetic acid solution. 4) followed by incubation in pronase (1:100 in PBS) for 30 minutes.

246, 195-207. J. R. (1996b) Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study. Anat. rec. 246, 185-194. , Gundersen, K. (1989) Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J. Muscle Res. Cell. Mot. 10, 197205. H. (1983) Skeletal Muscle Adaptations: A Review. Equine Exercice Physiology. Snow, D. , Persson, S. G. , and Rose, R. J. ) Burlington Press, Cambridge, pp. 160-183. H. S. (1980) Muscle fibre type composition of a number of limb muscles in different types of horse.

And Thornton,J. (1980) Histochemical properties of muscle fibre types and enzyme activities in skeletal muscles of standardbred trotters of different ages. Equine vet. J. 12, 175-180. , Persson, S. G. B. and Thornton, J. (1983) Skeletal muscle characteristics of young standardbreds in relation to growth and early training. In: Equine Exercise Physiology. Snow, D. , Persson, S. G. , and Rose, R. ) Granta Editions, Cambridge, pp. 200210. , Lindholm, A. and Persson, S (1989) Muscular adaptation of horses during intensive training and detraining.

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