HIV Protocols by James R. Lane (auth.), Nelson L. Michael MD, PhD, Jerome H.

By James R. Lane (auth.), Nelson L. Michael MD, PhD, Jerome H. Kim MD (eds.)

In HIV Protocols, Nelson Michael and Jerome Kim have geared up a suite of state-of-the-art suggestions crucial for learning the molecular biology, virology, and immunology of the HIV virus. The tools disguise a large diversity of analysis pursuits, together with quantitation of viral genomes, HIV promoter functionality, B-cell epitope mapping, viral coreceptor utilization, and measurements of T-cell functionality. designated emphasis is accorded to the learn of these viral and host immune responses to an infection that would be severe to the layout of powerful preventive vaccines.
HIV Protocols is the 1st HIV equipment booklet to pay attention both on virology, molecular biology, and immunology, in addition to to include equipment on chemokine receptor constitution and serve as. finished and cutting-edge, the ideas specific the following offer an imperative framework for unraveling the molecular and immunological features of HIV an infection.




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HIV Protocols

In HIV Protocols, Nelson Michael and Jerome Kim have equipped a suite of state of the art strategies crucial for learning the molecular biology, virology, and immunology of the HIV virus. The equipment conceal a wide variety of study pursuits, together with quantitation of viral genomes, HIV promoter functionality, B-cell epitope mapping, viral coreceptor utilization, and measurements of T-cell functionality.

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There are at least three different classes of reporter vtruses. Perhaps the most commonly used encode an easily assayed enzyme such as chloramphenicol acetyl transferase (1) or luciferase (2,3). These viruses allow for rapid determination of the relative infection frequency m a culture However, because quantitatmg these enzymes involves lysmg the cells, these viruses cannot be used for experiments m which it IS necessary to count single infected cells; the data generated reflect relative amounts of reporter gene product in the entire culture.

Thornwood, NV 3. Methods 1 MT-2 of -1 phase 2 Virus cells are cultured m cRPM1 and split every 34 d to mamtam a cell density x lo6 cells/ml. , l-2 d after split). stocks are pre-tltered on donor PBMC to determine TCID&mL (see Note 2) MT-2 Phenotype Assay 25 3 On the day of the assay, MT-2 cells are counted and resuspended at 1 x lo6 cells/ mL, 5 x lo4 cells (50 J&) are ahquoted into a 96-well flat-bottom mtcrottter plate (duplicate wells per vnus) 4. Control and test viruses are thawed m a 37OC water bath and diluted to 200 TCID,,/SO pL m cRPM1 5.

Thornwood, NV 3. Methods 1 MT-2 of -1 phase 2 Virus cells are cultured m cRPM1 and split every 34 d to mamtam a cell density x lo6 cells/ml. , l-2 d after split). stocks are pre-tltered on donor PBMC to determine TCID&mL (see Note 2) MT-2 Phenotype Assay 25 3 On the day of the assay, MT-2 cells are counted and resuspended at 1 x lo6 cells/ mL, 5 x lo4 cells (50 J&) are ahquoted into a 96-well flat-bottom mtcrottter plate (duplicate wells per vnus) 4. Control and test viruses are thawed m a 37OC water bath and diluted to 200 TCID,,/SO pL m cRPM1 5.

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