Human Fungal Pathogens by Alex van Belkum (auth.), Professor Dr. Judith E. Domer,

By Alex van Belkum (auth.), Professor Dr. Judith E. Domer, Professor Dr. George S. Kobayashi (eds.)

Provided listed below are either studies and up to date result of stories with medically vital fungi. in the course of the quantity, striking mycologists deal with the ecological position of pathogenic fungi, how they reason sickness, their interactions with the immune method, and their responses to antifungal brokers. lots of the awarded effects are in keeping with molecular organic methods: components the place classical and descriptive tools are utilized also are included.

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RFLP was the first approach applied to study genetic polymorphism among strains. However, interpretation of complex banding patterns displayed in ethidium bromide-stained gels is difficult because of the large number of faint bands and the lack of resolution between bands of high molecular weight (Denning et al. 1990; Burnie et al. 1992; Lin et al. 1995). Combining RFLP with hybridization using repeated sequences provides considerably more accurate banding patterns and allows determination of genetic relatedness among strains.

It possesses peptide sequences and features characteristics of retroviruses and retrotransposons, notably homologous sequences for reverse transcriptase, RNAse Hand endonuclease. Comparison of the A. fumigatus peptide sequences with those of other LTR retrotransposons showed that Afutl is most closely related to the gypsy family of retrotransposons described in the fruitHy Drosophila. Recent characterization of four other 'A cloned sequences revealed the existence of Afut2, another degenerate gypsy-like retrotransposon, different from Afutl (Paris and Latge 2001).

J Clin Mierobiol 31:463-464 XU J, Vilgalys R, Mitchell TG (1999) Lack of genetic diver- sity between two geographieally diverse sampies of Candida albicans isolated from patients infected with human immunodeficiency virus. J Bacteriol181: 13691373 Yamamoto Y, Kohno S, Koga H, Kakeya H, Tomono K, Kaku M, Yamazaki T, Arisawa M, Hara K (1995) Random amplified polymorphie DNA analysis of clinieally and environmentally isolated Cryptococcus neoformans in Nagasaki. J Clin Mierobiol 33:3328-3332 Yates-Siilata K, Sander DM, Keath EJ (1995) Genetic diversity in clinieal isolates of the dimorphie fungus Blastomyces dermatitidis detected by a PCR-based random amplified polymorphie DNA assay.

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