Imaging of Nucleic Acids and Quantitation in Photonic by Xavier Ronot, Yves Usson

By Xavier Ronot, Yves Usson

Visualization of nuclear parts has further enormously to our figuring out of the nucleus and its chemistry. The fluorescence method is taken into account the present-day typical for comprehending nuclear association. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy offers new applied sciences in fluorescence microscopy and photograph cytometry, in addition to their makes use of in imaging and quantification fluorescence in in situ hybridization.

Written via researchers with vast adventure within the use of photonic microscopy in nucleic acid learn, the chapters study such themes as: fixation; nucleotide substitution; fluorochrome homes; chemically transformed nucleotides and techniques of incorporation; unmarried mobilephone gel electrophoresis (comet assay) to notice DNA lesions; fluorescent imaging for in situ learn of nuclear distribution and colocalization of molecules (FISH mapping); rationalization of the fluorescence microscope and light-weight captors; visualization and processing of three-D info units; photo acquisition and quantification, in addition to examples; and more.

The chapters have been every one written through professionals at the specific matters. In a two-column layout, the idea and protocol are put along reviews and observations because of their adventure. The understandable motives of ideas including the unique tactics make Imaging of Nucleic Acids and Quantitation in Photonic Microscopy integral for your knowing and alertness of those important suggestions.

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Additional info for Imaging of Nucleic Acids and Quantitation in Photonic Microscopy

Example text

1 Aim . . . . . . . . . . . . . . . . . . . . 2 Protocols . . . . . . . . . . . . . . . . . . 2 Study of DNA Repair . . . . . . . . . . . . . . . . . 1 Aim . . . . . . . . . . . . . . . . . . . . 2 Protocols . . . . . . . . . . . . . . . . . . 3 Apoptosis . . . . . . . . . . . . . . . . . . . . . 1 Aim . . . . . . . . . . . . . . .

1 INTRODUCTION The maintenance of DNA integrity is essential for cellular life. Consequently, different approaches are used to check it. Recent developments are based on separation techniques coupled with very sensitive detection systems (high-performance liquid chromatography, mass spectrometry, electrochemistry). They allow the detection and quantification of different DNA lesions. Nevertheless, these latter techniques are not adapted to the detection of DNA damage at the cellular level.

10 Acknowledgments . . . . . . . . . . . . . . . . . . . . . 1 INTRODUCTION The maintenance of DNA integrity is essential for cellular life. Consequently, different approaches are used to check it. Recent developments are based on separation techniques coupled with very sensitive detection systems (high-performance liquid chromatography, mass spectrometry, electrochemistry). They allow the detection and quantification of different DNA lesions.

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