Immunochemical Techniques - Part C by Nathan P. Kaplan, Nathan P. Colowick, John J. Langone, Helen

By Nathan P. Kaplan, Nathan P. Colowick, John J. Langone, Helen Van Vunakis

The significantly acclaimed laboratory normal, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. seeing that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this present day - really an important booklet for researchers in all fields of existence sciences

Show description

Read Online or Download Immunochemical Techniques - Part C PDF

Best automotive books

Europe's Automotive Industry on the Move: Competitiveness in a Changing World, Vol.32

The car is a massive pillar of the trendy worldwide financial system and considered one of Europe's key industries. There can hardly ever be any doubt concerning the very important position of this area as an engine for employment, development and innovation in Europe, and there are the most important demanding situations and possibilities forward. The authors make clear a huge variety of concerns — globalisation and restructuring, exchange and overseas direct funding, innovation, legislation, and coverage — and positioned a different specialize in the hot member states.

Illustrated Encyclopedia of Extraordinary Automobiles

This chunky layout, retro-feel encyclopedia stories a hundred and fifty of the main magnificent vehicles in motoring background from the earliest to experiments for the longer term. every one motor vehicle is illustrated and observed by means of informative textual content, a colourful quote, and a requisites field. allotted generously during the e-book are pleasant photographic spreads exhibiting automobiles which are general in their period.

Motor City muscle: the high-powered history of the American musclecar

This is often the high-performance story of what used to be absolutely the quickest, loosest period in car background. in the course of the Sixties and into the Seventies, America’s carmakers fought an unbridled warfare for highway supremacy. the soldiers ranged from gentle and agile Z/28 Camaros and Boss 302 Mustangs to big-block brutes just like the 440 highway Runner and degree I 455 Buick GS.

Additional resources for Immunochemical Techniques - Part C

Example text

Exp. Biol. Med. 47, 2 0 0 (1941). METHODS IN ENZYMOLOGY, VOL. 74 Copyright © 1981 by Academic Press, Inc. All rights of reproduction in any form reserved. ISBN 0-12-181974-4 [2] FLUORESCENCE EXCITATION TRANSFER IMMUNOASSAY 29 cence" procedures require incubation of a tissue section with antibody toward a specific tissue antigen. The antibody may itself be labeled with a fluorescent tag or more frequently is indirectly labeled by washing away the unlabeled material and incubating the tissue a second time with labeled antibody to the first antibody.

Sci. A. 46, 1470 (1960). 32 IMMUNOASSAY METHODS [2] Second, with currently available dye pairs energy transfer from a fluorescent donor bound randomly to antibodies would be inefficient. Not only would some of the donors be too remote from the binding site to be efficiently quenched, but donors bound to nonspecific IgG would further add to background fluorescence. For these reasons the most practical labeling configuration requires fluorescent donors ("fluorescers") bound to a relatively pure antigen and antibody-bound acceptors ("quenchers") that are not fluorescent at the wavelengths of the donor emission.

Although powerful homogeneous enzyme immunoassay methods for proteins have recently been reported, r the fluorescence excitation transfer immunoassay 8 (FETI) that is described in this chapter offers significant advantages in terms of simplicity and generality of its application to both small molecules and proteins. Assay Principle One of the simplest concepts for detection of antibody binding is to measure the increase in proximity of the antibody and antigen occasioned by the binding event. Dipole-dipole-coupled electronic energy transfer between two chromophores is ideally suited to this type of measurement.

Download PDF sample

Rated 4.86 of 5 – based on 26 votes