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4 μg of ß-ecdysone and kept in normal culture medium (E) or actinomycin solution (EA) for 48 hours. From Henrikson and Clever (1972). 60 GENETIC MECHANISMS OF AGING 5 10 4 t h INTERMOLT (Days) 2 3P PUPAL MOLT Fig. 1 Growth of the salivary gland of Chironomus during the last larval instar. Open circles refer to total glandular protein, including the secretory proteins of the glandular lumen; closed circles refer to only cellular proteins. Where both values are given the latter was estimated from the former assuming that on the average 40% of the total protein consists of secretory proteins (Darrow and Clever, 1970).
Chromosoma 12, 1. C. H. (1971). J. Biol. Chem. 246, 4131. T. (1967). J. Cell Biol. J32, 339. Clever, U. (1961). Chromosoma 12, 607. Clever, U. (1962a). Chromosoma 13, 385. Clever, U. (1962b). J_. Insect Physio!. £, 357. 55 ULRICH CLEVER Clever, U. (1963). Chromosoma 14, 651. Clever, U. (1969). Ex£. Cell Res. J55, 317. , Storbeck, I. L. (1972) (in preparation). Clever, U. and Storbeck, I. (1970). Biochim. Biophys. Acta 217, 108. Clever, U. , Storbeck, I. and Romball, C G . (1969). Exp. Cell Res.