By Bert W. O'Malley, Joel G. Hardman (Eds.)
The severely acclaimed laboratory usual, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. considering the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this day - really a necessary booklet for researchers in all fields of existence sciences
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Extra resources for Part E: Hormone Action
1. Mitosis. A variety of methods for analyzing changing mitotic indices have been described in order to determine the length of mitosis in synchronized cells. 7,14,16,17 T h e derivation of a simple and precise analy~4p. N. Rao and J. Engelberg, in "Cell Synchrony, Studies in Biosynthetic Regulatioa" (I. L. Cameron and G. M. ), p. 332. Academic Press, New York, 1966. Y. Doida and S. Okada, Cell Tissue Kinet. 5, 15 (1972). 1, H. Firket and P. Mahieu, Exp. Cell Res. 45, 11 (1966). 17G. Galavazi and I).
Engelberg, in "Cell Synchrony, Studies in Biosynthetic Regulatioa" (I. L. Cameron and G. M. ), p. 332. Academic Press, New York, 1966. Y. Doida and S. Okada, Cell Tissue Kinet. 5, 15 (1972). 1, H. Firket and P. Mahieu, Exp. Cell Res. 45, 11 (1966). 17G. Galavazi and I). Bootsma, Exp. Cell Res. 41,438 (1966). 50 T H E CELL N U C L E U S AND CELL DIVISION  sis follows. The method requires the measurement of mitotic index and cell number at intervals following addition of the chemical agent to synchronized cells.
Trypsin. 0 for 2-4 hours at 20 or 37 °. 0 at 37 ° for 5 minutes to 24 hours. 4. Papain. 5 with 5 mM cysteine at 37 ° for 2 hours. 4, for 1-4 hours at 37 °. 5. a-Chymotrypsin. 8 for 1-4 hours at 37 °. 6. Ribonuclease. 5 with NaOH for 1-4 hours at 20, 37, 40, or 60 °. 1%) in the same solutions for 1-48 hours (usually 1-4 hours) at 37 °, 38 °, or 54 ° (usually 37°). Some authors have added 20 mM Mg -~÷ to the solution. Note that RNase has an optimum activity at about 60 ° . 7. Deoxyribonuclease. 8, for 1-4 hours (usually 1 hour), at 20 ° or 37 °.