Plant Cell Culture by L. A. Anderson, J. D. Phillipson, M. F. Roberts (auth.)

By L. A. Anderson, J. D. Phillipson, M. F. Roberts (auth.)

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Jirku et al. 36~ have covalently bound Solanum cells to porous, high surface area, beads of polyphenylene oxide which had been pre-activated with glutaraldehyde. The absence of low molecular weight toxins and the mildness of the chemical reaction prevented the loss in viability which might be expected from direct reaction with cell wall components. The natural tendency of suspension cultured cells to aggregate has been exploited by Lindsey et al. 18~, who found that after introduction of particles of polyurethane foam into suspension, cultures of Capsicum or Daucus, the cells collected preferentially in the pores of the foam.

E) The uncoupling of growth and production phases is probably the most important benefit of cell immobilization. Since the biomass is retained in the reactor its continuous replenishment is not required. Thus, cells can be grown under optimized conditions in a single separate operation and then used in a continuous reactor at dilution rates well in excess of those which would cause wash-out in free cell systems. The resulting high fluid velocity permissible even with these slow growing cells not only increases the efficiency of mass transfer, supplying fresh nutrients, but more importantly sweeps away inhibitory products, creating a favourable concentration gradient across the cell membrane.

References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 38 39 40 4l 43 43 44 46 47 47 49 52 52 55 56 57 57 Immobilization of plant cells is still a young technology, but this review illustrates the potential it has for improving the production of phytochemicals. antage is discussed. The delicate nature of plant cells limits the methods which can be used for their immobilization but once bound, the cells can be used for both biotransformations and perhaps more importantly biosynthesis.

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