Therapeutic Monoclonal Antibodies by James W. Larrick, C. Borrebaeck

By James W. Larrick, C. Borrebaeck

A overview of healing antibodies that have advertisement strength. It discusses PCR cloning of Igs, immortalization by way of gene move, scale up construction, infectious illnesses, viruses, micro organism, parisites, inflammation/immunology, blood grouping, cells, cytokines, tumours and MDR.

Show description

Read or Download Therapeutic Monoclonal Antibodies PDF

Best nonfiction_13 books

Missionary Discourses of Difference: Negotiating Otherness in the British Empire, 1840–1900

Missionary Discourse examines missionary writings from India and southern Africa to discover colonial discourses approximately race, faith, gender and tradition. The publication is organised round 3 issues: relatives, affliction and violence, which have been key components of missionary main issue, and significant axes round which colonial distinction was once cast.

Remote Sensing Handbook - Three Volume Set: Land Resources Monitoring, Modeling, and Mapping with Remote Sensing

A quantity within the three-volume distant Sensing instruction manual sequence, Land assets tracking, Modeling, and Mapping with distant Sensing records the clinical and methodological advances that experience taken position over the past 50 years. the opposite volumes within the sequence are Remotely Sensed facts Characterization, class, and Accuracies, and distant Sensing of Water assets, failures, and concrete experiences.

Extra info for Therapeutic Monoclonal Antibodies

Sample text

Normally one minute ramp times were used between these temperatures. Ethidium bromide-stained 2 percent agarose gels were used to separate PCR fragments. ASYMMETRIC AMPLIFICATION. Twenty ILl of amplified cDNA were run in low-melt agarose gels. , (AT) means both A and T were present in equimolar amounts during the synthesis of a particular position. Eco RI (5 I end) and Hind III (3 I end) sites are underlined. Two extra Gs were added 5 I of the restriction endonuclease site to facilitate enzyme cleavage.

48, 4348-4353. McCune, J. , et al. (1988) Science 241, 1632-1639. , and Hofschneider, P. H. (1977) Proc. Natl. Acad. Sci. USA 74, 3642-3647. , and Lee, I. E (1989) Biotechniques 7, 14-16. Morrison, S. , Johnson, M. , Herzenberg, L. , and Oi, V. T. (1984) Proc. Natl. Acad. Sci. USA 81,6851-6855. Morrison, S. , and Oi, V. T. (1989) Adv. Immunol. 44, 65-92. Mosier, D. , Gulizin, R. , Baird, S. , and Wilson, D. B. (1988) Nature 335,256-259. , and Margolies, M. N. (1985) Mol. Immunol. 22,477-88. , and Wigzell, H.

S. Lee and colleagues (1987) have used the PCR technique to detect small numbers of follicular lymphoma cells carrying the (t14;18) translocation. Although the aim of this work was the rapid construction of therapeutic Mabs, the technique we describe has tremendous potential for the study of the immune response to infectious agents and their toxins. It is now possible to amplify and sequence immunoglobulin variable regions from small numbers of B cells directly obtained from lesions or acquired during the course of infection.

Download PDF sample

Rated 4.93 of 5 – based on 8 votes