By Delmas J. Allen, Pietro M. Motta, Liberato J. A. DiDio
3 Dimensional Microanatomy of Cells and Tissue Surfaces makes a speciality of using scanning electron microscopy within the examine of the microanatomy of cells and tissues, cellphone relationships, and complicated organic relationships.
The choice first elaborates at the technical points of stereoprojection for electron microscopy; third-dimensional microanatomy of intracellular constructions; microcirculation stories through the injection-replica process with distinctive connection with portal circulations; and three-d structure of the mammalian liver. Discussions specialise in the guidance of vascular casts, portal circulations of varied organs, scanning electron microscopy, copying and printing stereopair negatives, stereoprojection, and excessive voltage electron microscopy. The textual content then takes a glance at scanning electron microscope bloodvessel casts research, 3 dimensional microanatomy of reticular tissues, kidney glomerular epithelium in keeping with assorted physiological states and experimental stipulations, and mammalian renal papilla and pelvis.
The manuscript examines the lung in scanning electron microscopy and stereopresentation, floor topography of endocardial endothelium, scanning electron microscopy of endothelium, human vas deferens, and seminal vesicles, and dynamic morphology of the apical membrane of lactating cells seen by means of freeze-fracture.
The choice is a invaluable reference for researchers drawn to using scanning electron microscopy within the research of the microanatomy of cells and tissues and organic relationships.
Read Online or Download Three dimensional microanatomy of cells and tissue surfaces : proceedings of the Symposium on Three Dimensional Microanatomy held in Mexico City, Mexico, August 17-23, 1980 PDF
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Additional resources for Three dimensional microanatomy of cells and tissue surfaces : proceedings of the Symposium on Three Dimensional Microanatomy held in Mexico City, Mexico, August 17-23, 1980
The fenestrated and frequently overlapping arrangement of the sinusoidal wall in some instances was interrupted by slender extensions of cells morphologically distinct from the thin endothelial cells which correspond to macrophages. Sinusoids: Kupffer cells Under the SEN the Kupffer cells were found to be much different from the endothelial cells lining the liver sinusoids (Figs. 7,8 and 9 ) . In all the specimens observed transition cellular types between Kupffer and endothelial 7, 24 cells were never found.
Space of Disse Under the SEM, the spaces of Disse were easily demonstrable across the openings [fenestrae) of the sinusoidal wall. They appeared as large pericapillary lacunae, into which microvilli formed from facing liver cells were proje cted. As seen topographically with the SEM, the extensions of these spaces appeared more numerous than classically reported in the light and TEM literatu re (Figs. 3,4 and 6 K The SEM images typically showed sinusoidal extensions between the surfaces of cells adjacent to the capillary.
These channels, earlier recognized by Steiner, were limited by the junctional complexes of the bile canaliculi and extended elsewhere in all directions clearly to connect adjacent subendothelial spaces 14 of Disse. Therefore the topography of the bile canaliculi, sinusoids and spaces of Disse permits one to distinguish in the hepatocyte three different faces. Some faces front the sinusoidal wall, some the bile canaliculi and others intercellular spaces. '^'^ It was also suggested^'^ that the hepatocytic surfaces directly facing the sinusoidal wall may have a different functional significance from the surfaces facing the intercellular spaces.