Tissue Culture of Trees by John H. Dodds (auth.), John H. Dodds (eds.)

By John H. Dodds (auth.), John H. Dodds (eds.)

1 John H. Dodds The tradition offragmen ts of plant tissue isn't really a very new technology, in reality as in the past as 1893 Rechinger (1893) defined the formation of callus on remoted fragments of stems and roots. The tradition of plant tissues in vitro on a nutrient medium used to be played through Haberlandt (1902), notwithstanding, his makes an attempt have been unsuccessful simply because he selected too basic a medium that lacked severe development elements. during the last fifty years there was a surge of improvement in plant tissue tradition recommendations and a number of strategies are actually avail­ capable (Dodds and Roberts, 1982). the main components are as follows. Callus tradition Callus is a slightly ill-dermed fabric. yet is generally defined as an un­ organised proliferating mass of tissue. even though callus cultures have loads of capability within the biotechnological elements of tissue tradition, i.e. secondary product formation, they aren't very appropriate for plant propagation. the main reason behind their unsuitability is that genetic aber­ rations happen in the course of mitotic divisions in callus progress (D'amato.l965). The aberrations might be of a big sort, similar to aneuploidy or endo­ reduplication. It follows hence that the genetic prestige of the re­ generated crops isn't the same as that of the mother or father sort. mostly phrases this genetic instability is bad, yet there are times whilst a callus degree could be purposely integrated to diversify the genetic base of the crop.

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Guineensis x E. melanococca. With continued subculture a fast-growing callus (FGC) was formed which had a FW doubling time of 10-20 days or 30-60 days depending on source. The FGC were granular and friable, and had dense areas of meristematic cells amongst larger vacuolated cells and lacunae. FGC can now be established in 6 -12 months, and of the 61 trees placed in culture between 1978 and 1980, 22 have now produced FGC. Embryogenesis of the FGC was obtained by transfer to a medium 34 Culture Propagation of Coconut, Date and Oil Palm with both auxin and cytokinin (Hanower and Pannetier, 1982), and by subculture the embryoids were multiplied or grown on into plantlets.

Z. J. , 7, 199-206 Reinert, J. (1956) 'Dissociation of Cultures from Picea glauca into Small Tissue Fragments and Single Cells', Science, 123,457-8 Schmidt, A. (1924) 'Ueber di Chlorophyllbild im Koniferenembryo', Bot. , 5,260-82 Slankis, V. E. (1975) 'Differentiation of Adventitious Buds on Douglas Fir EmbryosIn Vitro', Proc. Int. Plant Prop. E. S. A. ) Plant Tissue Culture Methods and Applications, Academic Press, New York, pp. E. L. (1974) 'Plantlet Formation in Pine Tissue Cultures', Am. J.

Two possible pathways of embryogenesis have been described for coffee which may be relevant to observations on the palms. Sandahl et al. (1977, 1979) have shown that embryoids formed on callus whilst it is on the primary culture medium are few in number: this is designated as LFSE (low frequency somatic embryogenesis); when transferred to the secondary medium, an embryogenic callus is produced which yields large numbers of embryoids: this is HFSE (high frequency somatic embryogenesis). The HFSE route is slower, but produces more embryos.

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