By Philip D. Reid (Eds.)
Tissue Printing explains and compiles step by step equipment and purposes of this elegantly basic and functional approach. The protocols should be simply transformed via the study biologist or instructor to review a wide selection of organic difficulties for easy learn or school room instructing. Tissue printing calls for no dear apparatus for profitable implementation, is secure, and will be used for either plant and animal structures. This useful laboratory advisor comprises many illustrative halftones, a whole bibliography, approach overviews, targeted protocols, and pattern useful applications. Read more...
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Extra resources for Tissue Printing. Tools for the Study of Anatomy, Histochemistry, And Gene Expression
2. All instructions for making the tissue print should be strictly followed because double images will be obtained if the tissue section is not carefully blotted and removed from the nitro cellulose filter. Detection of the AP-conjugated second antibody on tissue prints was selected over the peroxidase-conjugated second antibody procedure because the substrates used for detect ing the peroxidase, such as O-phenylenediamine and H 0 , 2 2 3 2 / Gladys I. Cassab are capable of detecting endogenous peroxidase activity in plant tissue sections (Cassab, Lin, Lin, & Varner, 1988).
Cell wall proteins unrelated t o extensin have been shown t o accumulate in response t o a variety of developmental and stress signals (Bozart & Boyer, 1987; Franssen, Nap, Gloudemans, Stiekema, & Van Dam, 1987; Tierney, Weichert, & Pluymers, 1988; Datta, Schmidt, & Marcus, 1989; Hong, Nagao, & Key, 1989; Keller & Lamb, 1989). Some plant cells are 26 / Gladys I. Cassab rich in glycine (Varner & Cassab, 1986). A petunia gene encoding a protein that is 67% glycine has been characterized (Condit & Meagher, 1987).
2 Μ CaCI ; this transfer does, in fact, occur. The simple procedure (Cassab & Varner, 1987) that follows al lows the immunolocalization of cell wall proteins, and the results cor relate with those of conventional light microscopy methods (Fig. 1). 2 In soybean seeds, extensin is primarily localized in the seed coat, hilum, and vascular bundles of the cotyledon. In soybean hypocotyls, however, the pattern of extensin distribution varies according t o the developmental stage of the tissue and the tissue type (Cassab, Lin, Lin, & Varner, 1988).